Fall Lab Course: BIOT-T425 Labs in Macromolecular Production

Fall Lab Course: BIOT-T425 Labs in Macromolecular Production

Dr. Lingling Chen, Associate Professor of Molecular and Cellular Biochemistry

Proteins are the cellular workhorse, and they are the targets for drug design. Obtaining the purified proteins is the first step…

At BioT T425/T525, you will use:

– bacteria, yeast, and mammalian cells to express protein

– various methods to purify proteins

– … and you will learn how to design and select:

  • Ÿ  vectors and host cells to express protein
  • Ÿ  procedures to purify protein
  • Ÿ  techniques to assay protein function and structure

Mondays (Lecture) 2:30pm- 3:15pm – Tuesdays and Thursdays (Lab) 1-3:30 pm

Prerequisites: BIOL-L211 and CHEM-C341, and one of the following: CHEM-C343, BIOL-T315, M255, M360, L319, L323, L324, L373 or M435 or talk to Dr. Chen

Counts as an upper-level lab for Biology majors